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Do not neglect to multiply the observed concentrations by any dilution factor of the original sample. Results are reported at Gallic Acid Equivalent, GAE, because the phenols in wine contain mostly other phenols, and only small amounts of gallic acid. Reducing sugars such as glucose and fructose cause minor interferences and must be corrected. Sulfites also cause an interference but the magnitude is variable. Ascorbate is another interference. It has a relative mass response of 0. This assay is responsive to any reducing substance, so if applied to other types of samples, large errors could be encountered. Since the assay measures all phenolics, the choice of gallic acid as standard is based on the availability of a stable and pure substance, and gallic acid is both, and it is less expensive than other options.
In addition, the response to gallic acid has been shown to be equivalent to most other phenolics in wine on a mass basis. You MUST make up your own standard curve each time the analysis is run. Do not use this data as a standard curve. The second section will have less growth and a few isolated colonies, while the final section will have the least amount of growth and many isolated colonies. The sample is spread across one quadrant of a Petri dish containing a growth medium.
Bacteria need different nutrients to grow. This includes water, a source of energy, sources of carbon, sulfur, nitrogen, phosphorus, certain minerals, and other vitamins and growth factors. A very common type of media used in microbiology labs is known as agar , a gelatinous substance derived from seaweed. The nutrient agar has a lot of ingredients with unknown amounts of nutrients in them. On one hand, this can be a very selective media to use because as mentioned bacteria are particular.
If there is a certain nutrient in the media the bacteria could most certainly not grow and could die. On the other hand, this media is very complex. Complex media is important because it allows for a wide range of microbial growth. The bacteria growth can be supported by this media greatly due in part to the high amounts of nutrients. Choice of which growth medium is used depends on which microorganism is being cultured, or selected for. Dependent on the strain, the plate may then be incubated , usually for 24 to 36 hours, to allow the bacteria to reproduce. At the end of incubation there should be enough bacteria to form visible colonies in the areas touched by the inoculation loop.
Automated equipment is used at industrial level for streak plating the solid media in order to achieve better sterilization and consistency of streaking and for reliably faster work. While streaking manually it is important to avoid scratching the solid medium as subsequent streak lines will be damaged and non-uniform deposition of inoculum at damaged sites on the medium yield clustered growth of microbes which may extend into nearby streak lines. Bacteria exist in water, soil and food, on skin, and intestinal tract normal flora.
The assortment of microbes that exist in the environment and on human bodies is enormous. The human body has billions of bacteria which creates the normal flora fighting against the invading pathogens.
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